ChemSpider 2D Image | bisbenzimide | C27H28N6O

bisbenzimide

  • Molecular FormulaC27H28N6O
  • Average mass452.551 Da
  • Monoisotopic mass452.232452 Da
  • ChemSpider ID1420

More details:

Validated by Experts, Validated by Users, Non-Validated, Removed by Users

2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazol [German] [ACD/IUPAC Name]
2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazole [ACD/IUPAC Name]
2'-(4-Éthoxyphényl)-5-(4-méthyl-1-pipérazinyl)-1H,1'H-2,5'-bibenzimidazole [French] [ACD/IUPAC Name]
2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzimidazole
2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole
2'-(4-ethoxyphenyl)-6-(4-methylpiperazin-1-yl)-1H,3'H-2,5'-bibenzimidazole
2,5'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)- [ACD/Index Name]
245-691-1 [EINECS]
bisbenzimide
1234011 [Beilstein]
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Validated by Experts, Validated by Users, Non-Validated, Removed by Users

99KZS6CNZX [DBID]
Hoechst 33342 [DBID]
hoechst33342 [DBID]
Bio1_000386 [DBID]
Bio1_000875 [DBID]
Bio1_001364 [DBID]
Bio2_000401 [DBID]
Bio2_000881 [DBID]
bisBenzimide H 33342 [DBID]
bisBenzimide H 33342;HOE 33342;Hoechst-33342;Hoechst33342 [DBID]
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  • References
  • Experimental Physico-chemical Properties
  • Miscellaneous

    • Bio Activity:

      <p><strong><span style="text-decoration: underline;">Overview</span></strong></p> <p> </p> <p>Blue fluorescent DNA stain that is commonly used in fluorescent microscopy. It is frequently used as a nuclear stain to stain nuclei. It is excited by UV light.</p> <p>Hoechst 33342 is cell permeable and has greater cell permeability than <a title="Hoechst 33258 | DNA dye | Hello Bio" href="https://www.hellobio.com/hoechst-33258.html" target="_self">Hoechst 33258</a>. The stain can be used on both live and fixed cells and is often used as an alternative to <a title="DAPI | Blue Nuclear DNA stain | Hello Bio" href="https://www.hellobio.com/dapi.html" target="_self">DAPI</a>.</p> <p> </p> <p>Hoechst 33342 binds to the AT-rich regions of the minor grove in DNA which renders it specific for nuclear chromatin. Its fluorescent intensity depends on the DNA content, chromatin structure and the position of the cell within the cell cycle.</p> <p> </p> <p><strong><span style="text-decoration: underline;">Uses and applications</span></strong></p> <p style="margin-bottom: 2em;">There is little fluorescent overlap with other commonly used small-molecule fluorophores / fluorescent proteins that emit in the green / red range. This makes it suitable for a wide range of applications.</p> <p><span style="text-decoration: underline;">Counterstain</span><strong><span style="text-decoration: underline;"><br /></span></strong>Hoechst 33342 is commonly used as a counterstain in fluorescent imaging.</p> <p> </p> <p><span style="text-decoration: underline;">Cell cycle studies / Apoptosis</span></p> <p style="margin-bottom: 2em;">Hoechst 33342 can stain the condensed nuclei of apoptotic cells to allow the identification of chromatin condensation and fragmentation. It is commonly used with <a title="Propidium iodide | Cell viability dye | Hello Bio" href="https://www.hellobio.com/propidium-iodide.html" target="_self">propidium iodide</a> to distinguish normal/live apoptotic and dead cell populations. It can additionally be used in conjunction with <a title="Acridine Orange Stain | Hello Bio" href="https://www.hellobio.com/acridine-orange-stain.html" target="_self">arcidine orange</a> (AO) to distinguish apoptotic cells.</p> <p style="margin-bottom: 2em;">Incorporation of <a title="BrdU | Hello Bio" href="https://www.hellobio.com/brdu.html" target="_self">Brdu</a> into DNA has a quenching effect on Hoechst fluorescence. Hoechst 33342 is also used in combination with BrdU to monitor cell cycle progression.</p> <p><span style="text-decoration: underline;">Stem cells</span></p> <p style="margin-bottom: 2em;">Combination of the Hoechst 33342 stain with surface-marker phenotyping allows the characterisation of a sub-population of stem cells termed the ‘side population’ (SP).</p> Hello Bio HB0787
      <p><strong><span style=text-decoration: underline;>Overview</span></strong></p> <p></p> <p>Blue fluorescent DNA stain that is commonly used in fluorescent microscopy. It is frequently used as a nuclear stain to stain nuclei. It is excited by UV light.</p> <p>Hoechst 33342 is cell permeable and has greater cell permeability than <a href=https://www.hellobio.com/hoechst-33258.html title=Hoechst 33258 | DNA dye | Hello Bio target=_self>Hoechst 33258</a>. The stain can be used on both live and fixed cells and is often used as an alternative to <a href=https://www.hellobio.com/dapi.html title=DAPI | Blue Nuclear DNA stain | Hello Bio target=_self>DAPI</a>.</p> <p></p> <p>Hoechst 33342 binds to the AT-rich regions of the minor grove in DNA which renders it specific for nuclear chromatin. Its fluorescent intensity depends on the DNA content, chromatin structure and the position of the cell within the cell cycle.</p> <p>&nbsp;</p> <p><strong><span style=text-decoration: underline;>Uses and applications</span></strong></p> <p style=margin-bottom: 2em;>There is little fluorescent overlap with other commonly used small-molecule fluorophores / fluorescent proteins that emit in the green / red range. This makes it suitable for a wide range of applications.</p> <p><span style=text-decoration: underline;>Counterstain</span><strong><span style=text-decoration: underline;><br /> </span></strong>Hoechst 33342 is commonly used as a counterstain in fluorescent imaging.</p> <p>&nbsp;</p> <p><span style=text-decoration: underline;>Cell cycle studies / Apoptosis</span></p> <p style=margin-bottom: 2em;>Hoechst 33342 can stain the condensed nuclei of apoptotic cells to allow the identification of chromatin condensation and fragmentation. It is commonly used with <a href=https://www.hellobio.com/propidium-iodide.html title=Propidium iodide | Cell viability dye | Hello Bio target=_self>propidium iodide</a> to distinguish normal/live apoptotic and dead cell populations [5]. It can additionally be used in conjunction with <a href=https://www.hellobio.com/acridine-orange-stain.html title=Acridine Orange Stain | Hello Bio target=_self>arcidine orange</a> (AO) to distinguish apoptotic cells.</p> <p style=margin-bottom: 2em;>Incorporation of <a href=https://www.hellobio.com/brdu.html title=BrdU | Hello Bio target=_self>Brdu</a> into DNA has a quenching effect on Hoechst fluorescence.&nbsp;Hoechst 33342 is also used in combination with BrdU to monitor cell cycle progression.<strong><span style=text-decoration: underline;></span></strong></p> <p><span style=text-decoration: underline;>Stem cells</span></p> <p style=margin-bottom: 2em;>Combination of the Hoechst 33342 stain with surface-marker phenotyping allows the characterisation of a sub-population of stem cells termed the &lsquo;side population&rsquo; (SP).</p> Hello Bio HB0787
      Biochemicals & small molecules Hello Bio HB0787
      Blue fluorescent DNA stain. Cell permeable. Nuclear stain. Hello Bio HB0787
      DNA Stain MedChem Express HY-15559
      Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. MedChem Express http://www.medchemexpress.com/cp-809101.html, HY-15559
      Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. Hoechst 33342 is a cell dye for DNA.;IC50 Value: ;These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 ?C for at least six months when protected from light. For long-term storag MedChem Express HY-15559
      Imaging, dyes, stains & labels/DNA fluorescent stains Hello Bio HB0787
      Others MedChem Express HY-15559

Predicted data is generated using the ACD/Labs Percepta Platform - PhysChem Module, version: 14.00

Density: 1.3±0.1 g/cm3
Boiling Point: 725.9±70.0 °C at 760 mmHg
Vapour Pressure: 0.0±2.4 mmHg at 25°C
Enthalpy of Vaporization: 106.0±3.0 kJ/mol
Flash Point: 392.8±35.7 °C
Index of Refraction: 1.691
Molar Refractivity: 136.0±0.3 cm3
#H bond acceptors: 7
#H bond donors: 2
#Freely Rotating Bonds: 5
#Rule of 5 Violations: 0
ACD/LogP: 4.63
ACD/LogD (pH 5.5): 1.01
ACD/BCF (pH 5.5): 1.00
ACD/KOC (pH 5.5): 4.93
ACD/LogD (pH 7.4): 3.35
ACD/BCF (pH 7.4): 168.26
ACD/KOC (pH 7.4): 1068.28
Polar Surface Area: 73 Å2
Polarizability: 53.9±0.5 10-24cm3
Surface Tension: 61.2±3.0 dyne/cm
Molar Volume: 355.2±3.0 cm3

Predicted data is generated using the US Environmental Protection Agency�s EPISuite™ 

                        
 Log Octanol-Water Partition Coef (SRC):
    Log Kow (KOWWIN v1.67 estimate) =  4.34

 Boiling Pt, Melting Pt, Vapor Pressure Estimations (MPBPWIN v1.42):
    Boiling Pt (deg C):  727.16  (Adapted Stein & Brown method)
    Melting Pt (deg C):  318.71  (Mean or Weighted MP)
    VP(mm Hg,25 deg C):  1.53E-017  (Modified Grain method)
    Subcooled liquid VP: 3.11E-014 mm Hg (25 deg C, Mod-Grain method)

 Water Solubility Estimate from Log Kow (WSKOW v1.41):
    Water Solubility at 25 deg C (mg/L):  0.2238
       log Kow used: 4.34 (estimated)
       no-melting pt equation used

 Water Sol Estimate from Fragments:
    Wat Sol (v1.01 est) =  1.5381 mg/L

 ECOSAR Class Program (ECOSAR v0.99h):
    Class(es) found:
       Aliphatic Amines
       Imidazoles

 Henrys Law Constant (25 deg C) [HENRYWIN v3.10]:
   Bond Method :   5.33E-020  atm-m3/mole
   Group Method:   Incomplete
 Henrys LC [VP/WSol estimate using EPI values]:  4.071E-017 atm-m3/mole

 Log Octanol-Air Partition Coefficient (25 deg C) [KOAWIN v1.10]:
  Log Kow used:  4.34  (KowWin est)
  Log Kaw used:  -17.662  (HenryWin est)
      Log Koa (KOAWIN v1.10 estimate):  22.002
      Log Koa (experimental database):  None

 Probability of Rapid Biodegradation (BIOWIN v4.10):
   Biowin1 (Linear Model)         :   0.2535
   Biowin2 (Non-Linear Model)     :   0.0036
 Expert Survey Biodegradation Results:
   Biowin3 (Ultimate Survey Model):   1.6313  (recalcitrant)
   Biowin4 (Primary Survey Model) :   2.6959  (weeks-months)
 MITI Biodegradation Probability:
   Biowin5 (MITI Linear Model)    :  -0.3973
   Biowin6 (MITI Non-Linear Model):   0.0003
 Anaerobic Biodegradation Probability:
   Biowin7 (Anaerobic Linear Model): -2.7031
 Ready Biodegradability Prediction:   NO

Hydrocarbon Biodegradation (BioHCwin v1.01):
    Structure incompatible with current estimation method!

 Sorption to aerosols (25 Dec C)[AEROWIN v1.00]:
  Vapor pressure (liquid/subcooled):  4.15E-012 Pa (3.11E-014 mm Hg)
  Log Koa (Koawin est  ): 22.002
   Kp (particle/gas partition coef. (m3/ug)):
       Mackay model           :  7.23E+005 
       Octanol/air (Koa) model:  2.47E+009 
   Fraction sorbed to airborne particulates (phi):
       Junge-Pankow model     :  1 
       Mackay model           :  1 
       Octanol/air (Koa) model:  1 

 Atmospheric Oxidation (25 deg C) [AopWin v1.92]:
   Hydroxyl Radicals Reaction:
      OVERALL OH Rate Constant = 331.5367 E-12 cm3/molecule-sec
      Half-Life =     0.032 Days (12-hr day; 1.5E6 OH/cm3)
      Half-Life =    23.229 Min
   Ozone Reaction:
      No Ozone Reaction Estimation
   Fraction sorbed to airborne particulates (phi): 1 (Junge,Mackay)
    Note: the sorbed fraction may be resistant to atmospheric oxidation

 Soil Adsorption Coefficient (PCKOCWIN v1.66):
      Koc    :  1.933E+006
      Log Koc:  6.286 

 Aqueous Base/Acid-Catalyzed Hydrolysis (25 deg C) [HYDROWIN v1.67]:
    Rate constants can NOT be estimated for this structure!

 Bioaccumulation Estimates from Log Kow (BCFWIN v2.17):
   Log BCF from regression-based method = 2.641 (BCF = 437.2)
       log Kow used: 4.34 (estimated)

 Volatilization from Water:
    Henry LC:  5.33E-020 atm-m3/mole  (estimated by Bond SAR Method)
    Half-Life from Model River: 2.337E+016  hours   (9.737E+014 days)
    Half-Life from Model Lake : 2.549E+017  hours   (1.062E+016 days)

 Removal In Wastewater Treatment:
    Total removal:              47.43  percent
    Total biodegradation:        0.46  percent
    Total sludge adsorption:    46.97  percent
    Total to Air:                0.00  percent
      (using 10000 hr Bio P,A,S)

 Level III Fugacity Model:
           Mass Amount    Half-Life    Emissions
            (percent)        (hr)       (kg/hr)
   Air       6.33e-006       0.774        1000       
   Water     3.73            4.32e+003    1000       
   Soil      92.4            8.64e+003    1000       
   Sediment  3.84            3.89e+004    0          
     Persistence Time: 8.41e+003 hr

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